Effect of cooling on sperm motility before and after frozen-thawed stallion semen

The aim of this study was to assess the effect of cooling on sperm motility before and after frozen-thawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5[degrees]C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5[degrees]C for 0, 2, 7 or 24 h) after the semen was frozen-thawed. Sperm progressive motility (83.1, 78.7, 74.8 or 70.3%, respectively) was significantly different (P