Responses of carp hepatopancreatic 7-ethoxyresorufin-O-deethylase and glutathione-dependent enzymes to organic pollutants-a field study

Modulations of hepatopancreatic activities of cytochrome P4501A (CYP1A) and glutathione‐dependent enzymes were investigated in carp collected in five ponds with different levels of contamination. The CYP1A‐dependent 7‐ethoxyresorufin‐O‐deethylase activity was markedly induced by polycyclic aromatic hydrocarbons present in the sediment at a total concentration of 0.9 mg/kg. Even a low organic contamination increased some of the glutathione‐dependent enzymatic activities, namely cytosolic glutathione reductase, glutathione S‐transferase toward 1‐chloro‐2,4‐dinitrobenzene, ethacrynic acid and l,2‐epoxy‐3‐(p‐nitrophenoxy)propane, and microsomal glutathione S‐transferase. These parameters should be considered as potential tools for the biomonitoring of exposure to chemicals and/or impacts of exposure. An example of a multivariate cluster and discriminant analysis of the obtained analytical and biochemical data proved to be very effectiv tools for the characterization of the level of contamination.