A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs...

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Veröffentlicht in:	Analyst (London) 2015-02, Vol.140 (4), p.999-1003
Hauptverfasser:	Funabashi, Hisakage, Shigeto, Hajime, Nakatsuka, Keisuke, Kuroda, Akio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors - genetics Cell Line DNA, Single-Stranded - chemistry Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - chemistry Glucose Transporter Type 1 - genetics Homeodomain Proteins - genetics Mice Molecular Sequence Data RNA, Messenger - analysis RNA, Messenger - genetics Transcription Factor HES-1
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Beschreibung
Zusammenfassung:	A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/c4an02064b