Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity

Strain JXJ 0135 T , an anti-cyanobacterial actinomycete, was isolated from a soil sample collected from Lushan Mountain, south China, and identified by using polyphasic approach. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0135 T belongs to the genus Streptomyces and exhibited distinct subclade and also highest similarity (98.6%) to Streptomyces scopuliridis RB72 T . The strain developed well-branched substrate and aerial mycelia, and produced spiral spore chains. Spores were elliptical and the spore surface was smooth. The strain contained LL -diaminopimelic acid with whole-cell sugars of mannose, rhamnose, glucose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphotidylinositol dimannoside, an unidentified aminophospholipid and an unknown phospholipid. The menaquinones were MK-9(H 6 ) and MK-9(H 8 ). The major components of the fatty acids were anteiso-C 15:0 , iso-C 16:0 , anteiso-C 17:0 , iso-C 15:0 , C 16:0 , iso-C 17:0 and iso-C 14:0 . The G+C content was 69.3 mol%. The DNA–DNA hybridization value between JXJ 0135 T and S. scopuliridis RB72 T was 41.2±1.4%. On the basis of the polyphasic data, strain JXJ 0135 T represents a novel species of the genus Streptomyces , for which the name Streptomyces lushanensis sp. nov. is proposed. The type strain is JXJ 0135 T (=DSM 42121 T =JCM 19628 T =KCTC 29261 T =KACC 17834 T =NRRL B-24994 T ).