MiR-378a-3p enhances adipogenesis by targeting mitogen-activated protein kinase 1

•MiR-378a-3p is up-regulated in adipose tissues of HFD-fed mice, as well as during the differentiation of 3T3-L1 preadipocytes.•MiR-378a-3p enhances adipogenesis in 3T3-L1 cells.•MiR-378a-3p induces adipogenesis by directly targeting MAPK1 which markedly decreases at the later stage of adipogenesis....

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Veröffentlicht in:Biochemical and biophysical research communications 2015-01, Vol.457 (1), p.37-42
Hauptverfasser: Huang, Nunu, Wang, Jiu, Xie, Weidong, Lyu, Qing, Wu, Jiangbin, He, Jie, Qiu, Wei, Xu, Naihan, Zhang, Yaou
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Sprache:eng
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Zusammenfassung:•MiR-378a-3p is up-regulated in adipose tissues of HFD-fed mice, as well as during the differentiation of 3T3-L1 preadipocytes.•MiR-378a-3p enhances adipogenesis in 3T3-L1 cells.•MiR-378a-3p induces adipogenesis by directly targeting MAPK1 which markedly decreases at the later stage of adipogenesis. Previous studies showed that miR-378a plays important roles in adipogenesis and obesity; however, the precise mechanisms of action remain unknown. Here, we found that miR-378a-3p expression is up-regulated in adipose tissues of high fat diet-induced obese mice, as well as during the differentiation of 3T3-L1 preadipocytes. Mir-378a-3p induced adipogenesis by targeting mitogen-activated protein kinase 1 (MAPK1). Overexpression of miR-378a-3p or silencing MAPK1 reduced MAPK1 expression and enhanced adipogenesis, whereas blockage of endogenous miR-378a-3p had the opposite effect, suggesting that miR-378a-3p promotes the adipogenesis of 3T3-L1 cells by targeting MAPK1.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2014.12.055