Residue analysis of fipronil and difenoconazole in okra by liquid chromatography tandem mass spectrometry and their food safety evaluation

•An LC–MS/MS method for fipronil and difenoconazole residues is reported in okra.•LOQ was 1ngg−1 for fipronil and its metabolites and 5ngg−1 for difenoconazole.•Fipronil and difenoconazole residues dissipated with PHI of 15 and 4days.•Washing and cooking treatments had significant effect on residue decontamination. A liquid chromatography tandem mass spectrometry (LC–MS/MS) based method is reported for simultaneous analysis of fipronil (plus its metabolites) and difenoconazole residues in okra. The sample preparation method involving extraction with ethyl acetate provided 80–107% recoveries for both the pesticides with precision RSD within 4–17% estimated at the limits of quantification (LOQ, fipronil=1ngg−1, difenoconazole=5ngg−1) and higher fortification levels. In field, both the pesticides dissipated with half-life of 2.5days. The estimated pre-harvest intervals (PHI) for fipronil and difenoconazole were 15 and 19.5days, and 4 and 6.5days at single and double dose of field applications, respectively. Decontamination of incurred residues by washing and different cooking treatments was quite efficient in minimizing the residue load of both the chemicals. Okra samples harvested after the estimated PHIs were found safe for human consumption.