Study of Saccharomyces cerevisiae Yeast Cells by Field-Flow Fractionation and Image Analysis

Asymmetrical flow field-flow fractionation has been applied to characterize Sac-vliaronivces cvrcvisiiw cells, the morphology of which has been assessed by image analysis during fermentation on glucose medium. Intact cells, budding or nonbud-ding, are eluted and separated according to the steric-hyp...

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Veröffentlicht in:Separation science and technology 1997-06, Vol.32 (9), p.1477-1492
Hauptverfasser: Pons, M. N., Litzén†, A., Kresbach, G. M., Ehrat, M., Vivier, H.
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Sprache:eng
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Zusammenfassung:Asymmetrical flow field-flow fractionation has been applied to characterize Sac-vliaronivces cvrcvisiiw cells, the morphology of which has been assessed by image analysis during fermentation on glucose medium. Intact cells, budding or nonbud-ding, are eluted and separated according to the steric-hyperlayer modes, while macromolecules and other cell debris are eluted according to the normal mode. † Present address: Astra Hassle AB, S-43183 Molndal, Sweden.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496399708004061