A trial proteomics fingerprint analysis of HepaRG cells by FD-LC-MS/MS

A proteomics profile analysis was performed on a human hepatocyte carcinoma cell line (HepaRG) by using the FD-LC-MS/MS method. One hundred and fifty-eight proteins were newly identified for the first time of which 10 were found to be specific to human hepatocytes. These proteins are a "proteom...

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Veröffentlicht in:	Analyst (London) 2015-01, Vol.140 (1), p.71-73
Hauptverfasser:	Nakata, Katsunori, Ichibangase, Tomoko, Saitoh, Ryoichi, Ishigai, Masaki, Imai, Kazuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotechnology Carcinoma, Hepatocellular - pathology Chromatography, Liquid Fingerprints Fluorescent Dyes - chemistry Human Humans Liver Neoplasms - pathology Neoplasm Proteins - analysis Peptide Mapping Proteins Proteomics Tandem Mass Spectrometry
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Beschreibung
Zusammenfassung:	A proteomics profile analysis was performed on a human hepatocyte carcinoma cell line (HepaRG) by using the FD-LC-MS/MS method. One hundred and fifty-eight proteins were newly identified for the first time of which 10 were found to be specific to human hepatocytes. These proteins are a "proteomics fingerprint" that can be used to characterize HepaRG cells.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/c4an01434k