A study of double stranded DNA adsorption on aluminum surface by means of electrochemical impedance spectroscopy
Immobilization of DNA on the solid surfaces is one of the goals in bio- and nano-technologies. Adsorption of double stranded DNA on the surface of aluminum was electrochemically studied by means of impedance spectroscopy. Nyquist diagram of aluminum in a tris (hydroxymethyl) ammoniummethane-HCl (Tri...
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Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-04, Vol.116, p.526-530 |
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Sprache: | eng |
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Zusammenfassung: | Immobilization of DNA on the solid surfaces is one of the goals in bio- and nano-technologies. Adsorption of double stranded DNA on the surface of aluminum was electrochemically studied by means of impedance spectroscopy. Nyquist diagram of aluminum in a tris (hydroxymethyl) ammoniummethane-HCl (Tris-HCl) buffer solution, pH 7.4 consisted of two overlapped capacitive semicircles. The high-frequency semicircle was related to the passivity of Cl(-)-containing aluminum species in the oxide layer, and low-frequency semicircle was attributed to metal dissolution. When DNA was added to the Tris-HCl buffer solution, Nyquist diagrams represented an inductive loop at low frequencies due to the adsorption of DNA on the pre-covered aluminum surface by hydroxy-contained species. The DNA adsorption on the aluminum surface was also confirmed by X-ray photoelectron spectroscopy. Open circuit potential variation with time also indicated the chemical adsorption of DNA on the aluminum surface. |
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ISSN: | 0927-7765 1873-4367 |
DOI: | 10.1016/j.colsurfb.2014.01.046 |