Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae

In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited ex...

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Veröffentlicht in:Talanta (Oxford) 2012-08, Vol.97, p.103-110
Hauptverfasser: Woo, Sung-Geun, Yoo, Keunje, Lee, Juyoun, Bang, Sara, Lee, Myungjin, On, Kwangchul, Park, Joonhong
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container_start_page 103
container_title Talanta (Oxford)
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creator Woo, Sung-Geun
Yoo, Keunje
Lee, Juyoun
Bang, Sara
Lee, Myungjin
On, Kwangchul
Park, Joonhong
description In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources. ► We added more explanations regarding the lack-of-fit tests and F-ratio. ► We added discussion about the differences of FA profiles among 3 microalgae. ► We corrected additional errors in terms, units or English usage as suggested.
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subjects Analysis methods
Analytical chemistry
Ankistrodesmus gracilis
Applied sciences
Biodiesel
Biofuels - microbiology
Biorefinery
Chemistry
Chlorella vulgaris
Chromatographic methods and physical methods associated with chromatography
Chromatography, Gas - methods
Culture
Exact sciences and technology
Fatty acids
Fatty Acids - analysis
Fatty Acids - isolation & purification
Gas chromatographic methods
Gas chromatography
Global environmental pollution
Microalgae
Microalgae - chemistry
Microalgae - growth & development
Microalgae - metabolism
Natural water pollution
Nitrogen limitation
Optimization
Pollution
PUFAs (polyunsaturated fatty acids)
Refining
Scenedesmus
Scenedesmus quadricauda
Species Specificity
Spectrometric and optical methods
Spectroscopy
Waste water
Waste Water - microbiology
Wastewater
Water treatment and pollution
title Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae
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