Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae
In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited ex...
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description | In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.
► We added more explanations regarding the lack-of-fit tests and F-ratio. ► We added discussion about the differences of FA profiles among 3 microalgae. ► We corrected additional errors in terms, units or English usage as suggested. |
doi_str_mv | 10.1016/j.talanta.2012.04.002 |
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► We added more explanations regarding the lack-of-fit tests and F-ratio. ► We added discussion about the differences of FA profiles among 3 microalgae. ► We corrected additional errors in terms, units or English usage as suggested.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2012.04.002</identifier><identifier>PMID: 22841053</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis methods ; Analytical chemistry ; Ankistrodesmus gracilis ; Applied sciences ; Biodiesel ; Biofuels - microbiology ; Biorefinery ; Chemistry ; Chlorella vulgaris ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Gas - methods ; Culture ; Exact sciences and technology ; Fatty acids ; Fatty Acids - analysis ; Fatty Acids - isolation & purification ; Gas chromatographic methods ; Gas chromatography ; Global environmental pollution ; Microalgae ; Microalgae - chemistry ; Microalgae - growth & development ; Microalgae - metabolism ; Natural water pollution ; Nitrogen limitation ; Optimization ; Pollution ; PUFAs (polyunsaturated fatty acids) ; Refining ; Scenedesmus ; Scenedesmus quadricauda ; Species Specificity ; Spectrometric and optical methods ; Spectroscopy ; Waste water ; Waste Water - microbiology ; Wastewater ; Water treatment and pollution</subject><ispartof>Talanta (Oxford), 2012-08, Vol.97, p.103-110</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-c12e4ad084011b8822d94783da93c4b118b5daca038bed422dc08917201edd1e3</citedby><cites>FETCH-LOGICAL-c527t-c12e4ad084011b8822d94783da93c4b118b5daca038bed422dc08917201edd1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2012.04.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26204514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22841053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woo, Sung-Geun</creatorcontrib><creatorcontrib>Yoo, Keunje</creatorcontrib><creatorcontrib>Lee, Juyoun</creatorcontrib><creatorcontrib>Bang, Sara</creatorcontrib><creatorcontrib>Lee, Myungjin</creatorcontrib><creatorcontrib>On, Kwangchul</creatorcontrib><creatorcontrib>Park, Joonhong</creatorcontrib><title>Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.
► We added more explanations regarding the lack-of-fit tests and F-ratio. ► We added discussion about the differences of FA profiles among 3 microalgae. ► We corrected additional errors in terms, units or English usage as suggested.</description><subject>Analysis methods</subject><subject>Analytical chemistry</subject><subject>Ankistrodesmus gracilis</subject><subject>Applied sciences</subject><subject>Biodiesel</subject><subject>Biofuels - microbiology</subject><subject>Biorefinery</subject><subject>Chemistry</subject><subject>Chlorella vulgaris</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Gas - methods</subject><subject>Culture</subject><subject>Exact sciences and technology</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - isolation & purification</subject><subject>Gas chromatographic methods</subject><subject>Gas chromatography</subject><subject>Global environmental pollution</subject><subject>Microalgae</subject><subject>Microalgae - chemistry</subject><subject>Microalgae - growth & development</subject><subject>Microalgae - metabolism</subject><subject>Natural water pollution</subject><subject>Nitrogen limitation</subject><subject>Optimization</subject><subject>Pollution</subject><subject>PUFAs (polyunsaturated fatty acids)</subject><subject>Refining</subject><subject>Scenedesmus</subject><subject>Scenedesmus quadricauda</subject><subject>Species Specificity</subject><subject>Spectrometric and optical methods</subject><subject>Spectroscopy</subject><subject>Waste water</subject><subject>Waste Water - microbiology</subject><subject>Wastewater</subject><subject>Water treatment and pollution</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhoMo7uzoT1ByEbxMm8_-OIkMqysseNFzqE6q1wzdnTZJ7zL_3gwz6nE9JZCnKlXvQ8gbzirOeP3hUGUYYc5QCcZFxVTFmHhGNrxt5E7qRj4nG8Zkt-u4YlfkOqUDK4Rk8iW5EqJVnGm5Ies-TAtEn8JMw0AHyPlIwXpHYYbxmHyiE-afwSU6hEghJUzJz_e09yHi4GeMhV-W0Vvo_ehLdWnzCCnjI2SM1K5j9g_l6ujkbQww3gO-Ii8GGBO-vpxb8uPzzff97e7u25ev-093O6tFk3eWC1TgWKsY533bCuE61bTSQSet6jlve-3AApNtj06VZ8vajjclEXSOo9yS9-e-Swy_VkzZTD5ZHEtwGNZkeK25EjXT-mmU1R3vpFbNf6AlZV0syYLqM1o2T6kEZpboJ4jHApmTR3MwF4_m5NEwZU6WtuTt5Yu1n9D9rfojrgDvLgAkC-MQYbY-_eNqwVTZrXAfzxyWmB88RpOsx9mi8xFtNi74J0b5DS1qv2c</recordid><startdate>20120815</startdate><enddate>20120815</enddate><creator>Woo, Sung-Geun</creator><creator>Yoo, Keunje</creator><creator>Lee, Juyoun</creator><creator>Bang, Sara</creator><creator>Lee, Myungjin</creator><creator>On, Kwangchul</creator><creator>Park, Joonhong</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope><scope>M7N</scope><scope>7QQ</scope><scope>7SR</scope><scope>7SU</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope></search><sort><creationdate>20120815</creationdate><title>Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae</title><author>Woo, Sung-Geun ; Yoo, Keunje ; Lee, Juyoun ; Bang, Sara ; Lee, Myungjin ; On, Kwangchul ; Park, Joonhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-c12e4ad084011b8822d94783da93c4b118b5daca038bed422dc08917201edd1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis methods</topic><topic>Analytical chemistry</topic><topic>Ankistrodesmus gracilis</topic><topic>Applied sciences</topic><topic>Biodiesel</topic><topic>Biofuels - microbiology</topic><topic>Biorefinery</topic><topic>Chemistry</topic><topic>Chlorella vulgaris</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Gas - methods</topic><topic>Culture</topic><topic>Exact sciences and technology</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - isolation & purification</topic><topic>Gas chromatographic methods</topic><topic>Gas chromatography</topic><topic>Global environmental pollution</topic><topic>Microalgae</topic><topic>Microalgae - chemistry</topic><topic>Microalgae - growth & development</topic><topic>Microalgae - metabolism</topic><topic>Natural water pollution</topic><topic>Nitrogen limitation</topic><topic>Optimization</topic><topic>Pollution</topic><topic>PUFAs (polyunsaturated fatty acids)</topic><topic>Refining</topic><topic>Scenedesmus</topic><topic>Scenedesmus quadricauda</topic><topic>Species Specificity</topic><topic>Spectrometric and optical methods</topic><topic>Spectroscopy</topic><topic>Waste water</topic><topic>Waste Water - microbiology</topic><topic>Wastewater</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woo, Sung-Geun</creatorcontrib><creatorcontrib>Yoo, Keunje</creatorcontrib><creatorcontrib>Lee, Juyoun</creatorcontrib><creatorcontrib>Bang, Sara</creatorcontrib><creatorcontrib>Lee, Myungjin</creatorcontrib><creatorcontrib>On, Kwangchul</creatorcontrib><creatorcontrib>Park, Joonhong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woo, Sung-Geun</au><au>Yoo, Keunje</au><au>Lee, Juyoun</au><au>Bang, Sara</au><au>Lee, Myungjin</au><au>On, Kwangchul</au><au>Park, Joonhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2012-08-15</date><risdate>2012</risdate><volume>97</volume><spage>103</spage><epage>110</epage><pages>103-110</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.
► We added more explanations regarding the lack-of-fit tests and F-ratio. ► We added discussion about the differences of FA profiles among 3 microalgae. ► We corrected additional errors in terms, units or English usage as suggested.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22841053</pmid><doi>10.1016/j.talanta.2012.04.002</doi><tpages>8</tpages></addata></record> |
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subjects | Analysis methods Analytical chemistry Ankistrodesmus gracilis Applied sciences Biodiesel Biofuels - microbiology Biorefinery Chemistry Chlorella vulgaris Chromatographic methods and physical methods associated with chromatography Chromatography, Gas - methods Culture Exact sciences and technology Fatty acids Fatty Acids - analysis Fatty Acids - isolation & purification Gas chromatographic methods Gas chromatography Global environmental pollution Microalgae Microalgae - chemistry Microalgae - growth & development Microalgae - metabolism Natural water pollution Nitrogen limitation Optimization Pollution PUFAs (polyunsaturated fatty acids) Refining Scenedesmus Scenedesmus quadricauda Species Specificity Spectrometric and optical methods Spectroscopy Waste water Waste Water - microbiology Wastewater Water treatment and pollution |
title | Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae |
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