Biological production of acetaldehyde from ethanol using non-growing Pichia pastoris whole cells

Acetaldehyde has been produced biologically using whole-cell Pichia pastoris an a semibatch fermentor. Ethanol and air were fed continuously, and the product, acetaldehyde, was removed by the air stream. Operation of the reactor exceeded 100 h, maintaining high alcohol oxidase activity. Low cell-mas...

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Veröffentlicht in:	Applied biochemistry and biotechnology 1991-01, Vol.28-29, p.513-525
Hauptverfasser:	HEIEN-KUN CHIANG, FOUTCH, G. L, FISH, W. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Pichia pastoris
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Zusammenfassung:	Acetaldehyde has been produced biologically using whole-cell Pichia pastoris an a semibatch fermentor. Ethanol and air were fed continuously, and the product, acetaldehyde, was removed by the air stream. Operation of the reactor exceeded 100 h, maintaining high alcohol oxidase activity. Low cell-mass concentration (9.9 g/L) minimized product inhibition. Ethanol concentration in the broth, oxygen concentration in the air, and pH were evaluated for their effects on the fermentation process.
ISSN:	0273-2289 1559-0291
DOI:	10.1007/BF02922630