Identification of environmental strains of Bacillus mycoides by fatty acid analysis and species-specific 16S rDNA oligonucleotide probe

Of 131 Gram-positive, aerobic spore-formers, isolated from a peat bog in North Germany, 34 strains were identified as Bacillus mycoides by fatty acid analysis. The identification of many strains as members of B. mycoides was surprising as these strains were non-motile and lacked the typical rhizoid...

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Veröffentlicht in:FEMS microbiology ecology 1997-11, Vol.24 (3), p.201-209
Hauptverfasser: von Wintzingerode, Friedrich, Rainey, Frederick A., Kroppenstedt, Reiner M., Stackebrandt, Erko
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Sprache:eng
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Zusammenfassung:Of 131 Gram-positive, aerobic spore-formers, isolated from a peat bog in North Germany, 34 strains were identified as Bacillus mycoides by fatty acid analysis. The identification of many strains as members of B. mycoides was surprising as these strains were non-motile and lacked the typical rhizoid colony morphology. The chemotaxonomic identification was supported by 16S rDNA analysis of a few strains and hybridization with a 16S rDNA oligonucleotide probe derived from sequence information of the type strain of B. mycoides. In addition, the identity of two isolates as strains of B. mycoides was confirmed by DNA-DNA hybridization. While fatty acid analysis and DNA-DNA hybridization confirmed the previously recognized close relatedness between the type strains of B. mycoides, B. cereus, B. thuringiensis and B. anthracis, the 16S rDNA probe was able to clearly discriminate B. mycoides strains from the type strains of the other three species.
ISSN:0168-6496
1574-6941
DOI:10.1016/S0168-6496(97)00057-3