Direct measurement of glucose profiles in immobilized yeast gels with a pH-insensitive micro-electrode under anaerobic conditions

A 10 mu m glucose sensor was developed based on a glucose oxidase coated Pt-electrode inserted in a capillary shaft. The internal buffer medium effected in a glucose response that was insensitive for the external pH. The sensor was successfully utilized at pH 4 under anaerobic conditions in gel part...

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Veröffentlicht in:	Biotechnology techniques 1993-03, Vol.7 (3), p.237-242
Hauptverfasser:	Cronenberg, C.C.H, Heuvel, J.C. van den, Ottengraf, S.P.P
Format:	Artikel
Sprache:	eng
Schlagworte:	anaerobic conditions Biological and medical sciences Biosensors Biotechnology electrodes Fundamental and applied biological sciences. Psychology glucose immobilization immobilized cells measurement Methods. Procedures. Technologies Saccharomyces cerevisiae Various methods and equipments
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Zusammenfassung:	A 10 mu m glucose sensor was developed based on a glucose oxidase coated Pt-electrode inserted in a capillary shaft. The internal buffer medium effected in a glucose response that was insensitive for the external pH. The sensor was successfully utilized at pH 4 under anaerobic conditions in gel particles containing homogeneously dispersed immobilized yeast cells.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF02566155