super(1)H- super(15)N-NMR studies of bacteriorhodopsin Halobacterium halobium : Conformational dynamics of the four-helical bundle

super(1)H- super(15)N-NMR studies of bacteriorhodopsin Halobacterium halobium : Conformational dynamics of the four-helical bundle

Series of uniformly and selectively super(15)N-labeled bacteriorhodopsins of Halobacterium halobium were obtained and a super(1)H- super(15)H-NMR study was performed in methanol/chloroform and NH sub(4)CHOO, medium which mimics that in the membrane in vivo. Less than half of the cross-peaks expected...

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Veröffentlicht in:European journal of biochemistry 1992-01, Vol.210 (1), p.223-229
Hauptverfasser: Orekhov, VYu, Abdulaeva, G V, Musina, LYu, Arseniev, A S
Format: Artikel
Sprache:eng
Schlagworte:
bacteriorhodopsin
conformation
Halobacterium salinarium
N.M.R
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Zusammenfassung:Series of uniformly and selectively super(15)N-labeled bacteriorhodopsins of Halobacterium halobium were obtained and a super(1)H- super(15)H-NMR study was performed in methanol/chloroform and NH sub(4)CHOO, medium which mimics that in the membrane in vivo. Less than half of the cross-peaks expected from the amino acid sequence of uniformly super(15)N-labeled bacteriorhodopsin were observed, using heteronuclear super(1)H- super(15)N coherence spectroscopy. The results clearly indicate that dynamic processes occur in the four helice bundle.
ISSN:0014-2956