Determination of Cyprinol and Cholic Acids in Bile and Muscle of Carp by Capillary Gas Chromatography

In recent years, many incidents of food poisoning by carp have been reported. It is important to determine the nature and amounts of the causative substances. Accurate determinations of cyprinol, one of the causative substances, and some bile acids were examined. Cyprinol and cholic acids were extracted from bile and muscle of carp. After alkalysis and derivatization of cyprinol, etc., analysis was carried out by FID-GC equipped with a fused silica methyl 50% phenylsilicone capillary column (25m×0.32mm i. d., 0.25μm film thickness). GC conditions were as follows: the sample was injected into solvent-cut injector and solvents were removed for 2 minutes at room temperature. The column temperature was programmed from 200°C to 260°C at 8°C/min. The injection temperature was 280°C. The detection limit of cyprinol was 5ng, and those of cholic acid, etc. were 3ng, based on the amount giving a signal equal to 3 times the amplitude of the baseline noise.