Restoration of rice husk-adsorbed lipase activity after rehydration
Hydrolytic and esterifying activities of lipase from Candida cylindracea adsorbed on rice husks were lost by dehydration using molecular sieve pellets and P sub(2)O sub(5) which removed 90-97% of the water after 9 days. However, only the esterifying activity of the enzyme was restored by gradual wat...
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Veröffentlicht in: | Biotechnology techniques 1998-05, Vol.12 (5), p.347-352 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Hydrolytic and esterifying activities of lipase from Candida cylindracea adsorbed on rice husks were lost by dehydration using molecular sieve pellets and P sub(2)O sub(5) which removed 90-97% of the water after 9 days. However, only the esterifying activity of the enzyme was restored by gradual water transfer via the organic phase to the dried enzyme either by direct water addition or by using salt hydrates as water donors. |
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ISSN: | 0951-208X 1573-6784 |
DOI: | 10.1023/A:1008862012156 |