Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus

The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were...

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Veröffentlicht in:	Journal of microbiological methods 2013-11, Vol.95 (2), p.251-252
Hauptverfasser:	Schubert, M., Stührk, C., Fuhr, M.J., Schwarze, F.W.M.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Basidiomycota - genetics Basidiomycota - growth & development Coculture Techniques - methods Gene Transfer Techniques Genetic Markers Luminescent Proteins - genetics Physisporinus vitreus Plasmids - genetics Red Fluorescent Protein Red fluorescent protein (RFP) Streptothricins Transformation Transformation, Genetic
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Zusammenfassung:	The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing red fluorescence protein activity. •Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.•Mitotically stable transformants were obtained showing red fluorescence protein activity.•The expression of the fluorescence marker allowed hyphal visualization in vivo.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2013.09.001