Detection of cytomegalovirus (CMV) by real-time PCR in fecal samples for the non-invasive diagnosis of CMV intestinal disease

Highlights • We assess the use of real-time PCR in feces for diagnosing CMV intestinal disease. • Qualitative fecal CMV PCR results are specific, making quantification dispensable. • Fecal PCR has a good specificity but low sensitivity for diagnosing CMV colitis. • CMV DNA detection in feces is asso...

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Veröffentlicht in:	Journal of clinical virology 2014-12, Vol.61 (4), p.517-522
Hauptverfasser:	Ganzenmueller, Tina, Kluba, Jeanette, Becker, Jan Ulrich, Bachmann, Oliver, Heim, Albert
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Aged, 80 and over Allergy and Immunology Biopsy Child Child, Preschool Cohort Studies Colon - virology Cytomegalovirus Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - virology Feces Feces - virology Humans Infant Infectious Disease Intestinal biopsy Intestinal disease Intestinal Diseases - diagnosis Intestinal Diseases - virology Male Middle Aged Molecular Diagnostic Techniques - methods Quantitative PCR Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Young Adult
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Beschreibung
Zusammenfassung:	Highlights • We assess the use of real-time PCR in feces for diagnosing CMV intestinal disease. • Qualitative fecal CMV PCR results are specific, making quantification dispensable. • Fecal PCR has a good specificity but low sensitivity for diagnosing CMV colitis. • CMV DNA detection in feces is associated with high CMV levels in gut biopsies.
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2014.10.009