Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM)

In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in orde...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2014-12, Vol.174 (7), p.2492-2503
Hauptverfasser:	Pérez-Lorenzo, E, Zuzuarregui, A, Arana, S, Mujika, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and medical sciences Biological Assay - methods Biology Biotechnology Chemistry Chemistry and Materials Science Crystals detection limit Endotoxins Endotoxins - analysis Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Quartz Quartz Crystal Microbalance Techniques - methods Toxins
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Beschreibung
Zusammenfassung:	In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.
ISSN:	0273-2289 1559-0291
DOI:	10.1007/s12010-014-1198-2