Coat Protein-mediated Resistance to Turnip mosaic virus in Chinese Cabbage (Brassica rapa ssp. pekinensis) by the Pollen-tube Pathway

Turnip mosaic virus of Chinese cabbage is the most destructive disease. Until now reliable sources of resistance and effective control methods are unavailable so development of highly resistant breeding lines is desirable. In this research we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The TuMV-CP gene was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid vector pBBBast-TuMV-CP (9.79 kb) which included TuMV-CP gene expression regulatory sequence (CaMV 35S promoter OCS terminator) the herbicide resistant Bar gene and other elements. Totally 0.15% of T0 plants showed positive for the gene TuMV-CP through PCR. Further PCR Southern blot analysis displayed that the gene TuMV-CP segments were transformed into T0 plants. Copyright 2014 Friends Science Publishers