Characterization of solubilized bradykinin B sub(2) receptors from smooth muscle and mucosa of guinea pig ileum

Characterization of solubilized bradykinin B sub(2) receptors from smooth muscle and mucosa of guinea pig ileum

Bradykinin (BK) B sub(2) receptors in guinea pig ileum were characterized in both membrane and soluble form. ( super(3)H)BK bound to a single class of sites with almost identical affinities in membranes prepared from the longitudinal muscle, circular muscle and mucosal layers of the ileum. The pharm...

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Veröffentlicht in:Biochemical pharmacology 1992-01, Vol.43 (8), p.1823-1827
Hauptverfasser: Ransom, R W, Young, G S, Schneck, K, Goodman, C B
Format: Artikel
Sprache:eng
Schlagworte:
biochemical characteristics
bradykinin B2
guinea-pigs
ileum
receptors
smooth muscle
solubilization
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Zusammenfassung:Bradykinin (BK) B sub(2) receptors in guinea pig ileum were characterized in both membrane and soluble form. ( super(3)H)BK bound to a single class of sites with almost identical affinities in membranes prepared from the longitudinal muscle, circular muscle and mucosal layers of the ileum. The pharmacology of the binding in the distinct layers was indistinguishable. The detergent CHAPS maximally solubilized nearly 80% of membrane binding activity in a very stable conformation.
ISSN:0006-2952