Sensitive and reproducible method for neutral monosaccharides and uronic acid determination in sugar beet pulp enzymatic treatment media and press juices by High Performance Anion Exchange Chromatography with pulsed amperometric detection

Sensitive and reproducible method for neutral monosaccharides and uronic acid determination in sugar beet pulp enzymatic treatment media and press juices by High Performance Anion Exchange Chromatography with pulsed amperometric detection

The resolution and the elution of arabinose, rhamnose, xylose, glucose, galactose, fructose, sucrose and galacturonic acid by an High Performance Anion Exchange Chromatography method were studied. The best results were obtained with a two step elution composed of a 25mM NaOH isocratic phase for 20 m...

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Veröffentlicht in:Biotechnology techniques 1998-02, Vol.12 (2), p.165-169
Hauptverfasser: GAILING, M. F, GUIBERT, A, COMBES, D
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
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Zusammenfassung:The resolution and the elution of arabinose, rhamnose, xylose, glucose, galactose, fructose, sucrose and galacturonic acid by an High Performance Anion Exchange Chromatography method were studied. The best results were obtained with a two step elution composed of a 25mM NaOH isocratic phase for 20 min followed by a 300mM sodium acetate gradient for 10 min. The reliability, repeatability and sensitivity of these analytical conditions were set.
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008852920291