Synthesis of oligosaccharides using immobilised 1,2-alpha-mannosidase from Aspergillus phoenicis: immobilisation-dependent modulation of product spectrum

A specific 1,2-alpha-mannosidase from A. phoenicis was immobilised on China clay, cellulose DE-52 and by entrapment in sodium alginate beads with respective yields of 19%, 16% and 18% in synthesis reactions consisting of 80% (w/w) mannose. Man alpha 1 leads to 6 man was the predominant product with China clay- and DE-52-immobilised enzymes with lesser amounts of man alpha 1 leads to 2 and man alpha 1 leads to 3. With the alginate bead-immobilised enzyme, man alpha 1 leads to 2 was the predominant linkage formed.