Development of indirect competitive ELISA for quantification of mitragynine in Kratom ( Mitragyna speciosa (Roxb.) Korth.)

Highlights • Monoclonal antibody against mitragynine was prepared. • The immunogens were prepared by the glutaraldehyde and carbodiimide methods. • The icELISA was used to determine mitragynine in Kratom leave samples.

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Bibliographische Detailangaben
Veröffentlicht in:	Forensic science international 2014-11, Vol.244, p.70-77
Hauptverfasser:	Limsuwanchote, Supattra, Wungsintaweekul, Juraithip, Keawpradub, Niwat, Putalun, Waraporn, Morimoto, Satoshi, Tanaka, Hiroyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Alkaloids Animals Antibodies, Monoclonal - immunology Aqueous solutions Chromatography Electrofusion ELISA Enzyme-Linked Immunosorbent Assay - methods Forensic science Forensic sciences High performance liquid chromatography icELISA Ionization Kratom Mass spectrometry Metabolites Methods Mice Mice, Inbred BALB C Mitragyna - chemistry Mitragynine Monoclonal antibody Pathology Plant Leaves - chemistry Proteins Quantitative analysis Secologanin Tryptamine Alkaloids - analysis Urine
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Beschreibung
Zusammenfassung:	Highlights • Monoclonal antibody against mitragynine was prepared. • The immunogens were prepared by the glutaraldehyde and carbodiimide methods. • The icELISA was used to determine mitragynine in Kratom leave samples.
ISSN:	0379-0738 1872-6283
DOI:	10.1016/j.forsciint.2014.08.011