Study of archaea community structure during the biodegradation process of nitrobenzene wastewater in an anaerobic baffled reactor

Nitrobenzene (NB) is an important industrial raw material in organic synthesis. However, successful biological treatment is challenging since NB wastewater is biologically toxic. During the experiment, the performance was examined during the acclimation process of NB in an anaerobic baffled reactor...

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Veröffentlicht in:International biodeterioration & biodegradation 2013-11, Vol.85, p.499-505
Hauptverfasser: Lin, Yingzi, Han, Xiangkui, Lu, Hai, Zhou, Jinliang
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Sprache:eng
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Zusammenfassung:Nitrobenzene (NB) is an important industrial raw material in organic synthesis. However, successful biological treatment is challenging since NB wastewater is biologically toxic. During the experiment, the performance was examined during the acclimation process of NB in an anaerobic baffled reactor (ABR). The removal efficiencies of NB and chemical oxygen demand were 98% and 90%, respectively. Furthermore, by applying polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) technology of 16SrDNA, this paper analyzes the structural change of the archaea community in the ABR before and after NB acclimation and identifies the dominant community. The sequence structure analysis of archaea 16S rDNA in DGGE profiles shows that after NB biodegradation, the archaea-dominant community primarily consists of Methanothrix soehngenii from Methanosarcina, Methanosaeta concilii from Methanosaeta, Methanobacterium beijingense 8-2, uncultured Archaeon TA04, and uncultured Methanobacterium sp. isolated from environmental samples, which may be the important functional archaea in an ABR for NB biodegradation. The study of the population structure distribution and the dominant archaea community is helpful for elucidating the mechanisms of the anaerobic biodegradation mechanism of NB. •NB removal efficiency in the initial stage and after domestication was 50% and 98%.•Archaea DGGE was analyzed in an ABR before and after NB biodegradation.•Archaea cluster and composition were analyzed before and after NB biodegradation.
ISSN:0964-8305
1879-0208
DOI:10.1016/j.ibiod.2013.05.017