Obtaining new composite biomaterials by means of mineralization of methacrylate hydrogels using the reaction–diffusion method

The present paper describes the synthesis and characterization of a new polymeric biomaterial mineralized with calcium phosphate using the reaction–diffusion method. The scaffold of this biomaterial was a hydrogel constituted by biocompatible polyethylene glycol methyl ether methacrylate (PEGMEM) and 2-(dimethylamino)ethyl methacrylate (DMAEM), which were cross-linked with N-N’-methylenebisacrylamide (BIS). The cross-linking content of the hydrogels was varied from 0.25% to 15% (w/w). The gels were used as matrix where two reactants (Na2HPO4 and CaCl2) diffused from both ends of the gel and upon encountering produced calcium phosphate crystals that precipitated within the polymer matrix forming bands. The shape of the crystals was tuned by modifying the matrix porosity in such a way that when the polymer matrix was slightly reticulated the diffusion reaction produced round calcium phosphate microcrystals, whilst when the polymer matrix was highly reticulated the reaction yielded flat calcium phosphate crystals. Selected area electron diffraction performed on the nanocrystals that constitute the microcrystals showed that they were formed by Brushite (CaHPO4.2H2O). This new composite material could be useful in medical and dentistry applications such as bone regeneration, bone repair or tissue engineering. [Display omitted] •New polymeric biomaterial mineralized with calcium phosphate using the reaction-diffusion method.-Growing of brushite nanocrystals within a polymeric matrix.•Mineralization by reaction diffusion method controls the crystal growth within gels.