High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm a...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biotechnology letters 1998-02, Vol.20 (2), p.113-116
Hauptverfasser:	Lee, Sang Jun, Kim, Il Chul, Kim, Dong Min, Bae, Kwang Hee, Byun, Si Myung
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biological and medical sciences Biotechnology E coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level Proteins
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!