High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm a...

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Veröffentlicht in:	Biotechnology letters 1998-02, Vol.20 (2), p.113-116
Hauptverfasser:	Lee, Sang Jun, Kim, Il Chul, Kim, Dong Min, Bae, Kwang Hee, Byun, Si Myung
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biological and medical sciences Biotechnology E coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level Proteins
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Zusammenfassung:	The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The amino-terminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005359920522