Total internal reflection (TIRF)-based quantification of procalcitonin for sepsis diagnosis – A point-of-care testing application

A new, highly sensitive fluorescence immunoassay for a TIRF (total internal reflection)-based point-of-care testing (POCT) device was developed for the detection of procalcitonin (PCT), a specific and early marker for sepsis and microbial infections. The immunoassay was performed on a bench-top syst...

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Veröffentlicht in:	Biosensors & bioelectronics 2014-09, Vol.59, p.251-258
Hauptverfasser:	Rascher, Daniela, Geerlof, Arie, Kremmer, Elisabeth, Krämer, Petra, Schmid, Michael, Hartmann, Anton, Rieger, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - chemistry Assaying Biological and medical sciences Biomarkers - blood Biosensing Techniques - instrumentation Biotechnology Blood Calcitonin - blood Calcitonin Gene-Related Peptide Cartridges Devices Equipment Design Evanescent field FIA Fundamental and applied biological sciences. Psychology hrPCT Human Humans Immunoassay Immunoassay - instrumentation Patients POCT Point-of-Care Systems Protein Precursors - blood Rats Recombinant Sensitivity and Specificity Sepsis Sepsis - blood Sepsis - diagnosis Tirf
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Beschreibung
Zusammenfassung:	A new, highly sensitive fluorescence immunoassay for a TIRF (total internal reflection)-based point-of-care testing (POCT) device was developed for the detection of procalcitonin (PCT), a specific and early marker for sepsis and microbial infections. The immunoassay was performed on a bench-top system that fulfilled all the necessary characteristics of a POCT application, including a short measurement time (
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2014.03.052