Extended map for the phaseolin linkage group of Phaseolus vulgaris L

The linkage relationship of 11 bean (Phaseolus vulgaris ) seed proteins (including phaseolin), 9 enzyme loci, and the P locus were analyzed in backcross and F sub(2) progenies by use of the software package "Mapmaker". The progenies were obtained by crossing the breeding line "XR-235-1" and the cultivar "Calima". Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity strains. The seed coat color of "Calima" is a red/beige mottled pattern and that of "XR-235-1" is white. Segregation at the P locus was followed by recording the phenotype of the BC sub(1)S sub(1) and F sub(3) seed. A linkage group comprising ca. 90 cM was detected with the following gene order: Est-2.-11-Pha-8-(Spe/Spg)-24-P-9-(Spa/Spb)-16-Spba-22-Mdh-1. In addition, another linkage group was detected: (Spd/Spf/Sph)-5-Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome.