Random amplified polymorphic DNA (RAPD) analysis in barley plants derived from anther culture

Random amplified polymorphic DNA (RAPD) was used to study DNA variation in 123 green, doubled-haploid plants derived from anther culture of six barley cultivars, including 'Andre', 'Harrington', 'Hector', 'Moravian III', 'Morex' and 'Otis'. Polymorphic fragments were found among derived lines and between cultivar and derived lines with 16 of 22 primers studied. The frequencies of polymorphism varied between derived lines. Lines derived from Moravian III showed polymorphism with 13 primers, while Hector and Hector derived lines were monomorphic with 22 primers studied. This indicated that DNA may be modified during anther culture and the degree of variation depended on the genotype of each cultivar.