Cloning and Expression of C4B Gene in Siji Goose

This study is aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank. A phylogenetic tree was constructed for homology analysis. The result shows that C4B gene in Siji goose shared relatively high homology with chicken and quail; while, Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and small intestine. In the present study, mRNA expression level of C4B gene in different tissues and organs of Siji goose was determined by fluorescence quantitative PCR, which provided basis for rapid diagnosis of specific animal diseases.