An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples
To improve the stool concentration procedure, we modified different steps of the standard formalin–ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin–ether concentration techniq...
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| Veröffentlicht in: | Journal of microbiological methods 2014-11, Vol.106, p.143-145 |
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| container_title | Journal of microbiological methods |
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| creator | Salleh, Fatmah Md Moktar, Norhayati Yasin, Azlin Mohd Al-Mekhlafi, Hesham M. Anuar, Tengku Shahrul |
| description | To improve the stool concentration procedure, we modified different steps of the standard formalin–ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin–ether concentration technique (M-FECT). Therefore, the M-FECT procedure provides enhanced detection of Cryptosporidium oocysts.
•M-FECT enhanced the detection of Cryptosporidium oocysts.•Seven samples were found positive with low level of Cryptosporidium oocysts.•M-FECT is applicable particularly in areas of low parasite endemicity. |
| doi_str_mv | 10.1016/j.mimet.2014.08.019 |
| format | Article |
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•M-FECT enhanced the detection of Cryptosporidium oocysts.•Seven samples were found positive with low level of Cryptosporidium oocysts.•M-FECT is applicable particularly in areas of low parasite endemicity.</description><subject>Adult</subject><subject>Aged</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Concentration technique</subject><subject>Cryptosporidiosis - diagnosis</subject><subject>Cryptosporidiosis - microbiology</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium oocysts</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Formalin–ether</subject><subject>Humans</subject><subject>Male</subject><subject>Microbiological Techniques - methods</subject><subject>Middle Aged</subject><subject>Parasitology - methods</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling - methods</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq2Kqiy0vwAJ-cglqT_iODn0sFrRFgmJS3u2HHtcvEri1HaQ9t_XsEuPcJrDPO-MZh6EriipKaHt1309-QlyzQhtatLVhPYf0IZ2klUdF_0Z2hRKVpJQdo4uUtoTQgVvuk_onAna86ZhGxS3M_bTEsMTWJxyCCM2YTYw56izDzMuLQN2jYBdiDg_AraQwbz0gsO7eFhySEuI3vp1wiGYQ8oJ-xk_RD3_wds0-tPgpKdlhPQZfXR6TPDlVC_R7--3v3Y_q_uHH3e77X1leM9yxaRhjurGOiOZc6InvOcD0VYPhjVOM8ME6wauG8Js33IqqZHWOSul4AMQfolujnPLCX9XSFlNPhkYRz1DWJOiLe8bybtWvo-Ktu0Y5UIUlB9RE0NKEZxaop90PChK1LMXtVcvXtSzF0U6VbyU1PVpwTpMYP9nXkUU4NsRgPKRJw9RJeOhiLA-lm8rG_ybC_4BIAqh8Q</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Salleh, Fatmah Md</creator><creator>Moktar, Norhayati</creator><creator>Yasin, Azlin Mohd</creator><creator>Al-Mekhlafi, Hesham M.</creator><creator>Anuar, Tengku Shahrul</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>201411</creationdate><title>An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples</title><author>Salleh, Fatmah Md ; Moktar, Norhayati ; Yasin, Azlin Mohd ; Al-Mekhlafi, Hesham M. ; Anuar, Tengku Shahrul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-27c2f1a4dfc72ff590393b0adabc24fa2c2528b3a402d963171c7dffd7753be03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Concentration technique</topic><topic>Cryptosporidiosis - diagnosis</topic><topic>Cryptosporidiosis - microbiology</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium oocysts</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Formalin–ether</topic><topic>Humans</topic><topic>Male</topic><topic>Microbiological Techniques - methods</topic><topic>Middle Aged</topic><topic>Parasitology - methods</topic><topic>Sensitivity and Specificity</topic><topic>Specimen Handling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salleh, Fatmah Md</creatorcontrib><creatorcontrib>Moktar, Norhayati</creatorcontrib><creatorcontrib>Yasin, Azlin Mohd</creatorcontrib><creatorcontrib>Al-Mekhlafi, Hesham M.</creatorcontrib><creatorcontrib>Anuar, Tengku Shahrul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salleh, Fatmah Md</au><au>Moktar, Norhayati</au><au>Yasin, Azlin Mohd</au><au>Al-Mekhlafi, Hesham M.</au><au>Anuar, Tengku Shahrul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2014-11</date><risdate>2014</risdate><volume>106</volume><spage>143</spage><epage>145</epage><pages>143-145</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>To improve the stool concentration procedure, we modified different steps of the standard formalin–ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin–ether concentration technique (M-FECT). Therefore, the M-FECT procedure provides enhanced detection of Cryptosporidium oocysts.
•M-FECT enhanced the detection of Cryptosporidium oocysts.•Seven samples were found positive with low level of Cryptosporidium oocysts.•M-FECT is applicable particularly in areas of low parasite endemicity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25193442</pmid><doi>10.1016/j.mimet.2014.08.019</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2014-11, Vol.106, p.143-145 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Aged Child Child, Preschool Concentration technique Cryptosporidiosis - diagnosis Cryptosporidiosis - microbiology Cryptosporidium Cryptosporidium - isolation & purification Cryptosporidium oocysts Feces - parasitology Female Formalin–ether Humans Male Microbiological Techniques - methods Middle Aged Parasitology - methods Sensitivity and Specificity Specimen Handling - methods |
| title | An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples |
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