An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples

To improve the stool concentration procedure, we modified different steps of the standard formalin–ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin–ether concentration techniq...

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Veröffentlicht in:	Journal of microbiological methods 2014-11, Vol.106, p.143-145
Hauptverfasser:	Salleh, Fatmah Md, Moktar, Norhayati, Yasin, Azlin Mohd, Al-Mekhlafi, Hesham M., Anuar, Tengku Shahrul
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Child Child, Preschool Concentration technique Cryptosporidiosis - diagnosis Cryptosporidiosis - microbiology Cryptosporidium Cryptosporidium - isolation & purification Cryptosporidium oocysts Feces - parasitology Female Formalin–ether Humans Male Microbiological Techniques - methods Middle Aged Parasitology - methods Sensitivity and Specificity Specimen Handling - methods
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Zusammenfassung:	To improve the stool concentration procedure, we modified different steps of the standard formalin–ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin–ether concentration technique (M-FECT). Therefore, the M-FECT procedure provides enhanced detection of Cryptosporidium oocysts. •M-FECT enhanced the detection of Cryptosporidium oocysts.•Seven samples were found positive with low level of Cryptosporidium oocysts.•M-FECT is applicable particularly in areas of low parasite endemicity.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2014.08.019