Examination of the pipetting variation between individuals performing random amplified polymorphic DNA (RAPD) analysis

The similarity between random amplified polymorphic DNA (RAPD) profiles generated by nine experimenters is presented. Despite simultaneously using the same pipettes, the same solutions and the same thermocycler, no two RAPD profiles generated identical banding patterns. This result suggests that bef...

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Veröffentlicht in:	Journal of microbiological methods 1998-05, Vol.32 (3), p.213-215
Hauptverfasser:	McEwan, Neil R., Bakht, Jehan, Cecchini, Edi, Coultart, Allan, Geri, Chiara, McDonald, Fiona M., McDonald, Morag S., Rahman, Nik Norulaini Ab, Williamson, Graham
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Diverse techniques Fundamental and applied biological sciences. Psychology Molecular and cellular biology Random amplified polymorphic DNA (RAPD) profile Simultaneous experiment Thermocycle
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Beschreibung
Zusammenfassung:	The similarity between random amplified polymorphic DNA (RAPD) profiles generated by nine experimenters is presented. Despite simultaneously using the same pipettes, the same solutions and the same thermocycler, no two RAPD profiles generated identical banding patterns. This result suggests that before using a particular primer, it is essential that all experimenters should obtain a reference profile for their own work, rather than comparing it to one generated by another researcher.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/S0167-7012(97)00086-9