Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, an...

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Veröffentlicht in:	Revista da Sociedade Brasileira de Medicina Tropical 2012-03, Vol.45 (2), p.257-259
Hauptverfasser:	Lino de Silva, Joao Guilherme, da Silva, Thiago Miranda, de Figueiredo Peloso, Eduardo, Lins Machado-Coelho, George Luiz, Mayrink, Wilson, Franco Ariosa, Marilia Caixeta, de Faria e Silva, Paulo Marcio, Marques, Marcos Jose
Format:	Artikel
Sprache:	eng
Schlagworte:	Leishmania
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Zusammenfassung:	INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p
ISSN:	0037-8682 1678-9849
DOI:	10.1590/S0037-8682201200020002