The study of the complexes of nitromedicine with cytochrome c and NO-containing aqueous dosage form in the wound treatment of rats

•Nitrosyl and NO-complexes are formed from nitromedicine and cytochrome c.•The increase of complexation of cytochrome c with nitromedicine in the presence of sodium ascorbate is shown.•Burn wound regenerative treatment of rats by nitromedicine-cytochrome c complexes was shown.•The improve vasodilatation (microcirculation level, heart rhythm variability) was estimated. The interaction of cytochrome c with nitromedicines, such as 5-nitrofural, 5-nitroxoline, metronidazole and sodium nitrite which enables the generation of nitric oxide or nitrosyl complexes in the presence of ascorbic acid or sodium ascorbate in acid medium has been investigated. The pharmaceutical compositions containing cytochrome c and nitromedicine complexes as active substances were studied in the experiments by using rats. It has been shown that positive local and systemic effects were estimated when NO-containing gel was used at burn treatment. These positive effects at the local level are due to a sufficient microcirculation index which indicates intensification of the blood flow in the microvessels in the injured area. These effects at the systemic level provide maintenance of the general heart rhythm and gradual recovery of the vegetative balance which is not observed in the animals of the control group.