Composition of polyphenol and polyamide compounds in common ragweed (Ambrosia artemisiifolia L.) pollen and sub-pollen particles

Ambrosia pollen and sub-pollen particles extracts were characterized by UHPLC-MS/MS. Main components of the extracts are flavonoid glycosides and spermidine derivatives, with sub-pollen particles extract containing significantly less flavonoids. [Display omitted] •Phenolic compounds were isolated from A. artemisiifolia pollen and sub-pollen particles.•Antioxidative properties and detailed composition of the extracts were determined.•Main flavonoid components are glycosides of quercetin, isorhamnetin and kaempferol.•Sub-pollen particles extract contains less flavonoid compounds than pollen extract.•Spermidine derivatives were detected in extracts of both pollen and sub-pollen particles. Phenolic composition of Ambrosia artemisiifolia L. pollen and sub-pollen particles (SPP) aqueous extracts was determined, using a novel extraction procedure. Total phenolic and flavonoid content was determined, as well as the antioxidative properties of the extract. Main components of water-soluble pollen phenolics are monoglycosides and malonyl-mono- and diglycosides of isorhamnetin, quercetin and kaempferol, while spermidine derivatives were identified as the dominant polyamides. SPP are similar in composition to pollen phenolics (predominant isorhamnetin and quercetin monoglycosides), but lacking small phenolic molecules (