Anticancer efficacy of photodynamic therapy with hematoporphyrin-modified, doxorubicin-loaded nanoparticles in liver cancer

•We developed novel nanoparticles (HP-NPs) to improve the therapeutic effect of PDT.•The in vitro phototoxicity was enhanced in HP-NPs compared with free HP.•The in vivo anticancer efficacy was markedly improved by HP-NPs-based PDT.•HP-NPs represent a potentially effective co-delivery system of HP a...

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Veröffentlicht in:Journal of photochemistry and photobiology. B, Biology Biology, 2014-11, Vol.140, p.49-56
Hauptverfasser: Chang, Ji-Eun, Yoon, In-Soo, Sun, Ping-Li, Yi, Eunjue, Jheon, Sanghoon, Shim, Chang-Koo
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Sprache:eng
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Zusammenfassung:•We developed novel nanoparticles (HP-NPs) to improve the therapeutic effect of PDT.•The in vitro phototoxicity was enhanced in HP-NPs compared with free HP.•The in vivo anticancer efficacy was markedly improved by HP-NPs-based PDT.•HP-NPs represent a potentially effective co-delivery system of HP and DOX. Photodynamic therapy (PDT) in combination with chemotherapy has great potential for cancer treatment. However, there have been very few attempts to developing cancer-targeted co-delivered systems of photosensitizers and anticancer drugs. We developed hematoporphyrin (HP)-modified doxorubicin (DOX)-loaded nanoparticles (HP-NPs) to improve the therapeutic effect of PDT in treating liver cancer. HP is not only a ligand for low density lipoprotein (LDL) receptors on the hepatoma cells but also a well-known photosensitizer for PDT. In vitro phototoxicity in HepG2 (human hepatocellular carcinoma) cells and in vivo anticancer efficacy in HepG2 tumor-bearing mice of free HP and HP-NPs were evaluated. The in vitro phototoxicity in HepG2 cells determined by MTT assay, annexin V-FITC staining and FACS analysis was enhanced in HP-NPs compared with free HP. Furthermore, compared with free HP-based PDT, in vivo anticancer efficacy in HepG2 tumor-bearing mice was markedly improved by HP-NPs-based PDT. Moreover, in both cases, the therapeutic effect was increased according to the irradiation time and number of PDT sessions. In conclusion, the HP-NPs prepared in this study represent a potentially effective co-delivery system of photosensitizer (HP) and anticancer drug (DOX) which improved the effects of PDT in liver cancer.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2014.07.005