effects of injection of amphetamine on female insemination in black blow fly, Phormia regina (Diptera: Calliphoridae)

An earlier study on the blowfly Phormia regina (Meigen) demonstrated that the injection of amphetamine (12 micrograms) depletes biogenic amine levels in the CNS. In the present study, P. regina females were injected with amphetamine (12 micrograms), each female was placed with three males and insemination success was determined. Amphetamine inhibited female insemination by 43.3% at 2-90 min post-injection and by 70% at 10-60 min post-injection. At 180-270 min post-injection, there was no significant inhibition of female insemination. This study indicates a possible role in insects for the biogenic amines in female insemination.