Skin biopsy as a nondestructive tool for the toxicological assessment of endangered populations of pinnipeds: Preliminary results on mixed function oxidase in Otaria flavescens

The aim of this study was to develop and validate a method for assessing the toxicological risk of endangered populations of pinnipeds based on a nondestructive biological tool, the skin biopsy specimen. Skin biopsies can be obtained from pinnipeds by anaesthetising the animals and taking a small am...

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Veröffentlicht in:	Chemosphere (Oxford) 1997-10, Vol.35 (8), p.1623-1635
Hauptverfasser:	Fossi, M.C., Savelli, C., Marsili, L., Casini, S., Jimenez, B., Junin, M., Castello, H., Lorenzani, J.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Applied ecology Argentina Biological and medical sciences Biomarkers - analysis Biopsy Caniformia - metabolism Cytochrome P-450 Enzyme System - analysis Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Environmental Exposure Female Fundamental and applied biological sciences. Psychology Isoenzymes - analysis Male Mammalia Marine Microsomes, Liver - enzymology Microsomes, Liver - metabolism Mixed Function Oxygenases - analysis Mixed Function Oxygenases - metabolism Nondestructive biomarkers OCs Otaria flavescens PAHs Pesticide Residues - analysis Pinnipeds Polycyclic Aromatic Hydrocarbons - analysis Polycyclic Aromatic Hydrocarbons - metabolism Seals, Earless - metabolism Seawater - chemistry Skin - enzymology Skin - pathology Skin biopsy Toxicity Tests Water Pollutants, Chemical - analysis
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Beschreibung
Zusammenfassung:	The aim of this study was to develop and validate a method for assessing the toxicological risk of endangered populations of pinnipeds based on a nondestructive biological tool, the skin biopsy specimen. Skin biopsies can be obtained from pinnipeds by anaesthetising the animals and taking a small amount of skin in the anterior flipper area, or by shooting a biopsy dart with a crossbow. Skin biopsy material is suitable for a wide range of chemical and biomarker analysis. Organochlorines and polycyclic aromatic hydrocarbons can be analysed in subcutaneous fat and MFO activity (BPMO), Cyt.P450 isoforms, and DNA damage can be detected in epidermis.
ISSN:	0045-6535 1879-1298
DOI:	10.1016/S0045-6535(97)00244-0