L-Lysine promotes the growth and secretion of hybridoma cell line AE9D6

L-Lysine (10 mu g/ml) promotes significant growth of hybridoma cell-AE9D6 for up to 9 weeks although mechanism of action was not evident. Maximum cell density (> 95% viability) of 1 X 10 super(8) cells /ml from a seeding density of 1 X 10 super(4) cells/ml was obtained within 36 h. Maximum cumula...

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Veröffentlicht in:	Biotechnology techniques 1997-09, Vol.11 (9), p.617-620
Hauptverfasser:	DATTA, D, KUNDU, P. K, SENTHILKUMAR, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Biological and medical sciences Biotechnology Cell hybridization, cell fusion, hybridomas Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies
Online-Zugang:	Volltext
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Zusammenfassung:	L-Lysine (10 mu g/ml) promotes significant growth of hybridoma cell-AE9D6 for up to 9 weeks although mechanism of action was not evident. Maximum cell density (> 95% viability) of 1 X 10 super(8) cells /ml from a seeding density of 1 X 10 super(4) cells/ml was obtained within 36 h. Maximum cumulative monoclonal antibody titre (between 120 h and 240 h) was 2.86 g/l.
ISSN:	0951-208X 1573-6784