Biotransformation of β-thujaplicin by cultured cells of Eucalyptus perriniana

Four β-thujaplicin glycosides were isolated from a cell suspension culture of Eucalyptus perriniana following administration of β-thujaplicin, and their structures determined. Four new biotransformation products, 4-isopropyltropolone 2-O-β- d-glucoside, 4-isopropyltropolone 2-O-β- d-gentiobioside, 6-isopropyltropolone 2-O-β- d-glucoside and 6-isopropyltropolone 2-O- d-gentiobioside, were isolated from cell suspension culture of Eucalyptus perriniana following administration of β-thujaplicin. The chemical structures of all products were determined by 1H and 13C NMR and MS spectroscopy. It was also observed that the production ratio of biotransformation products was higher in iron-deficient medium than in the medium containing iron ion.