Tetrahydroaminoacridine and related compounds interfere with fura-2 and indo-1

Tetrahydroaminoacridine and related compounds interfere with fura-2 and indo-1

In earlier experiments, using a fluorimetric method (fura-2), we found what seemed to be a decreased cytoplasmic [Ca 2+] in neuroblastoma cells when 1,2,3,4-tetrahydro-9-aminoacridine (THA) was added, but discovered by repeating our work under cell-free conditions, that THA affected the Ca 2+-fura-2...

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Veröffentlicht in:European journal of pharmacology 1992-01, Vol.225 (1), p.75-77
Hauptverfasser: Järlebark, Leif, Heilbronn, Edith
Format: Artikel
Sprache:eng
Schlagworte:
4-Aminopyridine (4-AP)
4-Aminopyridine - pharmacology
Animals
Biological and medical sciences
Dose-Response Relationship, Drug
Drug Interactions
Fura-2 (fluorimetry)
Fura-2 - analysis
Indoles - analysis
Medical sciences
Miscellaneous
Neuropharmacology
Pharmacology. Drug treatments
Rats
Tacrine
Tacrine - pharmacology
Tetrahydroaminoacridine (THA)
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Zusammenfassung:In earlier experiments, using a fluorimetric method (fura-2), we found what seemed to be a decreased cytoplasmic [Ca 2+] in neuroblastoma cells when 1,2,3,4-tetrahydro-9-aminoacridine (THA) was added, but discovered by repeating our work under cell-free conditions, that THA affected the Ca 2+-fura-2 signal. The present study aimed at investigating the previously observed interference of THA with fura-2. 1 nM THA completely inhibits fluorescence of fura-2 and another Ca 2+-indicator, indo-1, 1 μM each (apparent IC 50 = 40 μM) presumably by absorbing excitation light.
ISSN:0922-4106
0014-2999
DOI:10.1016/0922-4106(92)90042-T