A novel technique for entrapment of hybridoma cells in synthetic thermally reversible polymers

A new technique for entrapment of cells in thermally reversible polymer (poly-N-vinylcaprolactam - PVCl) has been developed. Using stabilizers, such as ovalbumin, sodium carboxymethylcellulose or egg powder were necessary to give structures that were stable upon stirring carriers. Two hybridoma line...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology techniques 1991-05, Vol.5 (3), p.223-226
Hauptverfasser:	MARKVICHEVA, E. A, KUZ'KINA, I. F, PASHKIN, I. I, PLECHKO, T. N, KIRSH, YU. E, ZUBOV, V. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology hybridoma immobilization Immobilization of organelles and whole cells Immobilization techniques immobilized cells Methods. Procedures. Technologies monoclonal antibodies poly-N-vinylcaprolactam polymers production supports
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A new technique for entrapment of cells in thermally reversible polymer (poly-N-vinylcaprolactam - PVCl) has been developed. Using stabilizers, such as ovalbumin, sodium carboxymethylcellulose or egg powder were necessary to give structures that were stable upon stirring carriers. Two hybridoma lines, in particular, 3B4 and 1A10 cells were entrapped in gel and produced monoclonal antibodies IgG and IgM classes, respectively, against Arabis mosaic virus.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00152786