Kinetic properties of cassava beta-glucosidase immobilized in photo-crosslinkable resins

Photo-crosslinkable resins (ENT-1000, ENT-2000 and ENT-4000) were used. Immobilized beta-glucosidase showed lower substrate affinity, better thermal stability, optimum pH range shifted towards the alkaline region and higher temperature optimum. beta-Glucosidase entrapped in ENT-4000 exhibited proper...

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Veröffentlicht in:	Biotechnology letters 1991-01, Vol.13 (10), p.697-700
1. Verfasser:	Yeoh, H.H
Format:	Artikel
Sprache:	eng
Schlagworte:	beta -glucosidase Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology heat stability immobilization Immobilization of enzymes and other molecules Immobilization techniques immobilized enzymes kinetics leaves Manihot esculenta Methods. Procedures. Technologies physicochemical properties resins substrates temperature
Online-Zugang:	Volltext
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Zusammenfassung:	Photo-crosslinkable resins (ENT-1000, ENT-2000 and ENT-4000) were used. Immobilized beta-glucosidase showed lower substrate affinity, better thermal stability, optimum pH range shifted towards the alkaline region and higher temperature optimum. beta-Glucosidase entrapped in ENT-4000 exhibited properties closest to those of the free enzyme.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF01088171