In Vitro Method of Studying the Angiotensin Activity of Chemical Compounds

We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selecti...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2014-11, Vol.158 (1), p.115-117
Hauptverfasser:	Spasov, A. A., Yakovlev, D. S., Bukatina, T. M., Brigadirova, A. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Angiotensin Angiotensin II Type 1 Receptor Blockers - pharmacology Animals Biomedical and Life Sciences Biomedicine Cell Biology Drug Evaluation, Preclinical Female In Vitro Techniques Inhibitory Concentration 50 Internal Medicine Laboratory Medicine Losartan - pharmacology Male Methods Muscle Contraction - drug effects Muscle, Smooth, Vascular - drug effects Pathology Portal Vein - drug effects Portal Vein - physiology Rats, Wistar Saralasin - pharmacology Vasoconstriction - drug effects
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Beschreibung
Zusammenfassung:	We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selective antagonist of type 1 angiotensin receptors) in concentrations of 10 −9 -10 −5 mol/liter. The half-maximal inhibitory concentrations (IC 50 ) of these substances were calculated.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-014-2705-8