Design of a stable cell line producing recombinant darbepoetin alpha based on CHO cells

A stable cell line that is based on CHO cells and produces 100 mg per liter of culture medium of recombinant darbepoetin alpha with a target glycosylated isoforms effective yield of about 30% has been selected. The expression product of the cell line was characterized and compared to the originator...

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Veröffentlicht in:	Applied biochemistry and microbiology 2014-12, Vol.50 (9), p.812-818
Hauptverfasser:	Shukurov, R. R, Lobanova, N. V, Savinova, I. N, Vorobyova, I. G, Nurbakov, A. A, Ermolina, L. V, Orlova, N. V, Mosina, A. G, Antonova, L. P, Khamitov, R. A, Seryogin, Yu. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biology Biomedical and Life Sciences cell lines Cellular biology culture media Gene expression Genetic Engineering glycosylation Life Sciences Medical Microbiology Microbiology Producers Selection
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A stable cell line that is based on CHO cells and produces 100 mg per liter of culture medium of recombinant darbepoetin alpha with a target glycosylated isoforms effective yield of about 30% has been selected. The expression product of the cell line was characterized and compared to the originator (Aranesp, Amgen). It was shown that the obtained preparation contained all isoforms characteristic of the originator. The created cell line can be used for the development of industrial cultivation and a purification scheme for recombinant darbepoetin alpha production.
ISSN:	0003-6838 1608-3024
DOI:	10.1134/S0003683814090063