NET formation induced by Pseudomonas aeruginosa cystic fibrosis isolates measured as release of myeloperoxidase–DNA and neutrophil elastase–DNA complexes

Highlights • Limited DNAseI digestion of NETs allows better reproducibility. • A modified MPO–DNA ELISA and a new HNE–DNA ELISA was used to quantify NETs. • Cystic fibrosis isolates of Pseudomonas aeruginosa trigger respiratory burst in human neutrophils. • Early P. aeruginosa cystic fibrosis isolates induce significantly more robust NET release than late isolates.