Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150–250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful to...

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Veröffentlicht in:	Journal of microbiological methods 2013-03, Vol.92 (3), p.244-245
Hauptverfasser:	Li, Shuxian, Ridenour, John B., Kim, Hun, Hirsch, Robert L., Rupe, John C., Bluhm, Burton H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Agrobacterium tumefaciens - genetics Agrobacterium-mediated transformation Ascomycota - genetics conidia Diaporthe longicolla Gene Transfer Techniques genomics GFP expression Glycine max - microbiology insertional mutagenesis Mutagenesis, Insertional - methods pathogens Phomopsis longicolla Phomopsis seed decay Plant Diseases - microbiology soybeans Transformation, Genetic
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Beschreibung
Zusammenfassung:	To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150–250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2012.12.015